Michael Lichten wrote:
>> In article <espinoza.859230092 at cgl.ucsf.edu>, espinoza at cgl.ucsf.edu> (Hernan Espinoza) wrote:
>> > Albert Spielmann <Albert.spielmann at bota.unine.ch> writes:
> >
> > >I'm working with the two-hybrid system from Clontech.
> > >The yeast strains are CG1945 and Y190. Both have a ura3-52 deletion.
> > >Normally they can't grow without uracil. But as said in the supplier's
> > >protocol, it must grow!! Why?
> >
> > Your question is a bit ambiguous, under what conditions must the
> > strains grow? I'm not familiar with the Clonetech system, but I would
> > wager it's to select for a URA3 marker on a plasmid...
> >
> > -Hernan
>> Let's clear up a misconception right now. ura3-52 is NOT a deletion; it's
> a Ty insertion in the URA3 coding region.
>> --
> Michael Lichten
>lichten at helix.nih.gov
These strains, as explained in the catalog, have URA+Gal-lacZ
constructions inserted into a chromosome.
--
Sanford Silverman >Opinions expressed here are my own<
American Cyanamid
silvermans at pt.cyanamid.com "Yeast is Best"
