In article <33368E33.41C6 at po.uni-stuttgart.de>, Martin Ligr
<martin.ligr at po.uni-stuttgart.de> wrote:
> Hello:
> I am currently using DAPI to visualize yeast nuclei, but the signal from
> nucleus is often obscured by strong signal from mitochondria. So I am
> thinking about using Hoechst 33258 or 33342. Could someone give me some
> info what is the difference between these two and how do they compare to
> DAPI? And is there any nucleus specific stain at all?
> Thanks,
> Martin Ligr
I believe that all, unfortunately, preferentially stain AT-rich sequences,
which is why the high mitochondrial staining. You may wish to try
propidium iodide, which in our hands gave much less mitochondrial
background.
--
Michael Lichten
lichten at helix.nih.gov
