Hi Fellow Netters,
We currently use the following recipy for out AA stocks (I think as per the
diffco manual). I am wondering if it would be a good idea to make a super mix
stock with all the amino acids ie., 12g lysine+ 8g His+ 12 g leu + 8 g trp and
then add 4 ml of the stock per l of media. Will there be a solubility problem
or some sort of incompatibility among the AAs if I do this??
Any input will be much appreciated.
Thanx very much
Amino acids (store stocks at 4o after filter sterilising)
Amino Amount added AA Stock solution
acid per liter media (per 400 ml)
L-lysine HCl 1 ml 12 g
L-histidine HCl 1 ml 8 g
L-leucine 2 ml 6 g
L-tryptophan 4 ml 2 g
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Karthikeyan Ramachandran Ph.D
Research Fellow, Rm. No. SA136
School of Biological and Chemical Sciences
Faculty of Science and Technology Phone (Off) 61 (3) 5227 1406
Deakin University Phone (Res) 61 (3) 5241 9806
Geelong, Victoria Fax 61 (3) 5227 2022
Australia 3217 e-mail karthi at deakin.edu.au
Home page http://www.deakin.edu.au/~karthi/
