Zymolyase doesn't dissolve too well in pure water-- I have to use 1M
sorbitol or sucrose to dissolve it up. I usually make a 2mg/ml stock
solution in 1M sorbitol and freeze aliquots. The sorbitol probably also
helps protect against damage during freezing. I don't know exactly how
much activity gets lost with freezing and thawing, but I've never had a
problem even after several freeze/ thaws.
-Daniel
dhs3 at cornell.edu
> Dear yeast workers,
>>> I am just starting to work with YAC libraries and I would like to know what
> is the best way to prepare stock solutions of zymolyase 100T that could be
> stored frozen.
>> None of the protocol books I have read describe how to prepare stock
> solutions. Is the enzyme stable in aqueous solution?
>> Thanks very much for your help.
>>>>>> Malcolm J. Gardner, Ph.D.
> The Institute for Genomic Research
> 9712 Medical Center Drive
> Rockville, MD 20850
>> Phone: (301)-838-3519
> FAX: (301)-838-0208
>> email: gardner at tigr.org
