Their is precedent for multiple translation initiation events in Saccharomyces:
Gillman, E. C., Slusher, L. B., Martin, N. C., and Hopper, A. K. (1991).
MOD5 translation initiation sites determine N6-isopentenyladenosine
modification of mitochondrial and cytoplasmic tRNA. Mol. Cell. Biol. 11,
2382-2390.
Consider the possibility of leaky scanning as well as internal initiation:
Yun, D. F., Laz, T. M., Clements, J. M., and Sherman, F. (1996). mRNA
sequences influencing translation and the selection of AUG initiator codons
in the yeast Saccharomyces cerevisiae. Mol. Microbiol. 19, 1225-1239.
Finally, consider that there may be additional transcripts that lack the
upstream start codon:
Natsoulis, G., Hilger, F., and Fink, G. R. (1986). The HTS1 gene encodes
both the cytoplasmic and mitochondrial histidine tRNA synthetases of S.
cerevisiae. Cell 46, 235-243.
>Hi Folks,
>I'm currently performing a Yeast one hybrid screen with Gal4-cDNA fusions.
>The so far pulled out clones are kind of weird but are making perfect
>sense when they are translated in a frame shift. Does anybody know if
>second site translation starts do happen in Yeast which might produce
>functional transcription factors?
>Hope to hear from you soon. Ciao, Matthias
>
-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR 97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs at admin.ogi.edu
