We need to choose an appropriate shuttle vector for a protein
complentation experiments. The major requirement is that the vector
should not have any BamHI and BglII sites, since we are going to use these
restrictases to create the constructs.
The vector should have:
bacterial origin of replication,
bacterial selectable marker (Amp or something)
yeast 2mu origin,
yeast selectable marker, preferrably Trp1,
f1 origin (optional)
If anyone knows of a plasmid that satisfies all these requirements please
let us know. Thanks for any help.