I want to digest the cell walls from my S. pombe. The protocols I have found
use Novozyme sometimes together wil Zymolase. The problem is Novozyme has
some protease activity, which is a bad thing because I want to do some
immunofluorescence on a plasma membrane protein I have expressed. Does
anyone know of any other enzymes, that have no protease activity, that I can
use? Can I use Zymolase alone?
Thanks in advance,