IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

URGENT Help !!!

Arturo Sánchez asanchez at cibnor.mx
Wed Jun 4 17:18:20 EST 1997


In recent days I finished some DNA extraction from some marine yeast 
strains, for my thesis work. I am using the electrophoretic patterns as a 
taxonomic tool to identifie marine yeast.
The problem is that I have both DNA genomic and mitochondrial. So, when I 
use some  restriction enzymes (Eco RI, Hinf I, Kpn I, Alu I, and Pst I) I 
can see that in my 2% agarose gel there is some bands, I mean there is 
some patterns, but the backgound is very "strong". Such a high background 
that the paterns are not completly clear. By the way, I must say that in 
a 0.8% gel I have a very clean DNA (only one band, no background).
I have read several articles and always the pictures are very nice, 
almost no background.
 The questions are: 

1) The background is the result of both kinds of DNA? Should I separete 
them by sacarose or CsCl gradients, and then make the restriction?

2) How can I make my gels cleaner? What should I do to have gels good 
enough for pictures?

I am close to finish my thesis, in fact this are my last experiments. So, 
I need an urgent help. I'll be very glad to receive some ideas. Please 
answer to asanchez at cibnor.mx

Thanks a lot.

Arturo Sanchez
Marine Pathology Unit.
Marine Yeast Laboratory.
Center for Biological Research.

La Paz, Baja California Sur. Mexico.




More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net