Michel Guilloton wrote:
>> Hello !
>> We're trying to select yeast mutants sensitive to calcofluor white. We
> purchased from Sigma the following product "F-3543 Fluorescent
> brightener 28". Sigma says that this is another name for Calcofluoir
> white M2R.
>> Maybe somebody could tell us whether this is a good stuff or not, and
> eventually where one could find out the genuine one that interacts with
> yeast cell wall.
>> Michel Guilloton
>>guilloton at unilim.fr
I used the same product to test a mutant I created and it worked out
fine. I only had the problem that pH 6,5 of the medium was too low for
solving the Calcofluor white (using a concentration of 1,5 mg/ml). Make
sure that your medium has a pH that is high enough to solve the
Calcofluor White and low enough that your yeast strain is still able to