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help for vortexing

Sanford Silverman silvermans at nospam.cyanamid.com
Wed Jul 30 14:08:15 EST 1997

Nistor Nicolaevici wrote:
> My goal is to detect various substances in the citosol of
> S.cerevisiae. To get maximum efficiency of the experiment I need to know
> the exact minimum period of time after which all the cells are disrupted
> using a Vortex with glass beads. Could you give me a hint about this ?
> *Do you know any MARKERS for S.cerevisiae intracellular compounds
> to signal the point when the plateau is reached?
> *Are there any microscopical methods to differentiate between
> the intact and ghost cells after coloration with METHYLENE-BLUE?
> *or do you think that the single possibility is to transfer the
> material on Sabouraud medium and look for the limit moment when the
> collonies dissapear?
>  Thankfully,
>  C. Diunzis
>  Biochemistry Dpt.
>  University of Medicine & Pharmacy, Timisoara Romania

Check them under phase contrast.  Lysed cells are phase dark.
Unlysed remain phase bright.

Good luck

Sanford Silverman               >Opinions expressed here are my own< 
American Cyanamid  
	REPLY TO:			"Yeast is Best"
silvermans at pt.cyanamid.com

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