Nistor Nicolaevici wrote:
>> My goal is to detect various substances in the citosol of
> S.cerevisiae. To get maximum efficiency of the experiment I need to know
> the exact minimum period of time after which all the cells are disrupted
> using a Vortex with glass beads. Could you give me a hint about this ?
>> *Do you know any MARKERS for S.cerevisiae intracellular compounds
> to signal the point when the plateau is reached?
> *Are there any microscopical methods to differentiate between
> the intact and ghost cells after coloration with METHYLENE-BLUE?
> *or do you think that the single possibility is to transfer the
> material on Sabouraud medium and look for the limit moment when the
> collonies dissapear?
>> C. Diunzis
> Biochemistry Dpt.
> University of Medicine & Pharmacy, Timisoara Romania
Check them under phase contrast. Lysed cells are phase dark.
Unlysed remain phase bright.
Sanford Silverman >Opinions expressed here are my own<
REPLY TO: "Yeast is Best"
silvermans at pt.cyanamid.com