My goal is to detect various substances in the citosol of
S.cerevisiae. To get maximum efficiency of the experiment I need to know
the exact minimum period of time after which all the cells are disrupted
using a Vortex with glass beads. Could you give me a hint about this ?
*Do you know any MARKERS for S.cerevisiae intracellular compounds
to signal the point when the plateau is reached?
*Are there any microscopical methods to differentiate between
the intact and ghost cells after coloration with METHYLENE-BLUE?
*or do you think that the single possibility is to transfer the
material on Sabouraud medium and look for the limit moment when the
University of Medicine & Pharmacy, Timisoara Romania