to whom it may concern:
I want to photograph some budding yeast cell, both GFP and
DAPI-stainded.fixing cell will reduce gfp'signal so i can't do it.staining
live cell with DAPI I cann't get insisting result, sometime is good some
time is bed.whould you please tell me what facts will effect the result of
DAPI stain and any suggestion? thank you very much.