I would like to use an auxotrophic marker which contains a small (1-3 base)
mutation in one of the well known marker genes for S. cerevisiae.
For example, the amber and ochre mutations would be fine for my purposes.
However, I have been unable to find the actual sequence changes present in
The strain I have on hand (YNN214) carries ura3-52, lys2-801 (amber) and
ade2-101 (ochre) alleles. Does anyone know the sequences changes present in
any of these alleles?
?Other suggestions for strains and markers that I could use? Again, it must
be a selectable marker with a small and known sequence change.