>> I have heard that there is a new strain for 2-hybrid screening which
> incorporates a third selection of positives, and leads to a significant
> reduction in "false" positives. Could anyone direct me to the appropriate
> paper for this?
See Genetics, 144, pp1425-1436, by Philip, et al. From the paper, it
appears that all you really need is the GAL2-ADE2 reporter, as that one
alone eliminated the majority of the false positives.Matthew
DuPont Central Research