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Protein-protein Interactions, Two-Hybrid System

Dr Ian Graham ian.graham at nottingham.ac.uk
Tue Jul 22 05:59:19 EST 1997

I am intending to study protein-protein interactions in S. cerevisiae and
S. pombe using a 2-Hybrid System. Unfortunately, I do not have a S.
cerevisiae library. Does anyone have or know of some place where I can get
a S. cerevisiae library which will interact with the pGBT9 (GAL4
DNA-binding domain) plasmid from Clontech? Does anyone also have the CG1945
yeast strain that they might be able to send me?

Robin Haw

Dept. of Genetics 
University of Nottingham
Queen's Medical Centre

Tel. (0115) 924 9924 Ext. 42596

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