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GFP photobleaching

msawyer at ZOO.UVM.EDU msawyer at ZOO.UVM.EDU
Mon Jul 21 13:49:13 EST 1997


Does anyone have experience with and/or know anything about 
photobleaching with GFP fusions?  Having difficulty observing cells 
for more than 10-20 sec. at a time before they turn brownish-green and 
faded.

Have tried using phenylenediamine in suspension mixture with no luck.
Any ideas would be greatly appreciated.



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