We are currently working with a GFP fusion proteins using the old version
of GFP that is not mutated. We also have a laser-scan confocal microscope
with 488 and 548 excitation wavelengths. WE want to counterstain with a
acid dye. Does anyone have any advice on which dye to use in combination
with the GFP-fusion protein?
Any help would be greatfully appreciated.
-Al (plummer at em.uni-frankfurt.de)