In article <hammers-2606971122300001 at haema101.gsf.de>, hammers at gsf.de
(Wolfgang Hammerschmidt) wrote:
> Hi folks,
>> I am wondering how big an insert in a S.c. CEN/ARS plasmid could be.
> Specifically, we would like to clone a big chunk of mammalian DNA (more
> than 100 kbp) on an E.coli F-factor plasmid (BAC) which could also
> replicate extrachromosomally in S.c. What are the requirements both for
> the ARS/CEN plasmid part and the specific yeast strain to keep such a
> shuttle plasmid in S.c. without becoming instable?
>> Thanks for sharing your knowledge!
Circular chromosome IIIs work fine, so I think your answer would be at
least 300 kb.
lichten at helix.nih.gov