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Fixing pombe for counting?

Michael Lichten lichten at helix.nih.gov
Mon Jul 7 08:48:18 EST 1997


In article <5pgnef$bo6$1 at oravannahka.Helsinki.FI>, Thomas O Westerling
<westerli at cc.helsinki.fi> wrote:

> Fixing the cells and counting in Burker cuvettes would be nice but I
> have way to many samples for that to be fast.
> A problem wich does not make my life any easier is that the cells
> aggregate easily. Their "hugging" is really a mess when counting.
> Sonication separates them but only temporary. Is there any chemical
> around to stop this?
> 
We have used both polypropylene glycol 2000 (Aldrich), 1/10e5 dilution and
TWEEN-80, 1/10e3 dilution, as an anti-clumping agents for cerevisiae.  

Michael Lichten

-- 
Michael Lichten
lichten at helix.nih.gov



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