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2-hybrid interactions in diploid cells

B. Drees drees at u.washington.edu
Sun Jul 6 19:14:06 EST 1997


	I am trying to detect  pairwise 2-hybrid interactions by mating.
The binding domain hybrids are in alpha cells and the activation domain
hybrids are in a cells, after mating diploids are screened for 2-hybrid
signal. Does anyone know if the ploidy of a cell might affect expression
of the hybrid proteins or the two-hybrid interaction?  The hybrid proteins
are constitutively expressed from CEN plasmids.  Thanks for your help!

Becky Drees

drees at u.washington.edu

   




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