In article <5pb1ol$5ok at mserv1.dl.ac.uk>, "Miguel A. Rodriguez Gabriel"
<MARODRIGUEZ at trasto.cbm.uam.es> wrote:
> Dear Yeasters,
>> I am trying to prepare minimal medium (YNB 6.7 g/l; glucose 20
> grow S. cerevisiae. I want to have a final concentration of 100 mM calcium in
> this medium, and for this purpose I add CaCl2. My problem is that when I add
> CaCl2, a white precipitate appears.
> Has any of you prepared this kind of medium? How did you solve this
> PRECIPITATION problem?
Replace NH4SO4 with NH4Cl (3.5 g/L) and use YNB-nitrogen source and amino