We'd like to try a version of a "three-hybrid" screen: interaction of
bait and prey is dependent on phosphorylation of bait, bait is not
phosphorylated in yeast, cDNA library would be screened for kinase that
What we need is a full-length mammalian cDNA expression library fused to
the SV40 nuclear localization signal, preferably on a 2u plasmid vector.
Does something like this actually exist? Any ideas?
John M. Sedivy, Ph.D.
Dep't Mol. Biol., Cell Biol. & Biochem.