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lacZ in sectoring assay

Dmitry Belostotsky dab at CNSVAX.ALBANY.EDU
Wed Jan 22 16:58:32 EST 1997


I am wondering if lacZ can be used as a marker of the plasmid loss
similarly to ade mutation as used in synthetic lethal screens. I am
doing it with ADE3 but my strain grows slowly and red color develops so
slowly it makes the whole thing almost impractical, even though
sectoring is good (when I can see it). Would lacZ be good for that
(cell autonomous and easy to detect)?
Thanks
DB

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Dmitry A. Belostotsky
Assistant Professor

Department of Biological Sciences
State University of New York at Albany
1400 Washington Ave.
Albany NY 12222
Tel. (518) 442 4368
FAX  (518) 442 4767
E-mail dab at cnsvax.albany.edu
http://barbara.bio.albany.edu/~dab/
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