Marguerite Evans wrote:
>> Dear Everyone,
>> Now the year is back into full swing, I have a question:
>> Is it possible to pH YEPD media between pHs 3 to 9 or thereabouts? I am
> aware that it has a strong buffering capacity, but am wondering if it is at all
> feasible. Would precipitation or protein hydrolysis of any sort occur? Any
> comments or advice would be most appreciated.
>> Marguerite Evans
> School of Biochemistry and Molecular Genetics
> UNSW Sydney 2052
>> voice: (+61)(2)385 2030
> fax: (+61)(2)313 6271
> email: p2158740 at acsusun.acsu.unsw.edu.au
This is easily done, as for killer medium. See the Cold Spring Harbor
lab manual for phosphate/citrate buffer, pH 4.5 or any recent killer
paper for a reference.
Sanford Silverman >Opinions expressed here are my own<
silvermans at pt.cyanamid.com "Yeast is Best"