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S. Silverman silvermans at pt.cyanamid.com
Mon Jan 20 18:43:07 EST 1997

Marguerite Evans wrote:
> Dear Everyone,
> Now the year is back into full swing, I have a question:
> Is it possible to pH YEPD media between pHs 3 to 9 or thereabouts?  I am
> aware that it has a strong buffering capacity, but am wondering if it is at all
> feasible.  Would precipitation or protein hydrolysis of any sort occur?  Any
> comments or advice would be most appreciated.
> Cheers,
> Marguerite.
> Marguerite Evans
> School of Biochemistry and Molecular Genetics
> UNSW  Sydney  2052
> Australia
> voice: (+61)(2)385 2030
> fax:   (+61)(2)313 6271
> email: p2158740 at acsusun.acsu.unsw.edu.au

This is easily done, as for killer medium.  See the Cold Spring Harbor
lab manual for phosphate/citrate buffer, pH 4.5 or any recent killer 
paper for a reference.

Sanford Silverman               >Opinions expressed here are my own< 
American Cyanamid  
silvermans at pt.cyanamid.com                      "Yeast is Best"

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