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How I get Mitos!!!

Michael Forte forte at ohsu.edu
Mon Jan 13 16:29:16 EST 1997


 In article <1997Jan10.172910 at crick.rz-berlin.mpg.de>,
jaensch at mpimg-berlin-dahlem.mpg.de wrote:

> I try to get yeast mitochondria. The removal of the cell wall with zymolyase
> doesnt work very well. Is the activity of the enzym depending on the growth
> conditions of the yeast cell? Which factors are important to optimize this
> step?
> 
> Thank you for helping me!
> 
> Lothar Jaensch, Universitaet Hannover

A variation on a commonly used protocal to isolate mitos from yeast can be
found in Methods in Enzymology Vol. 260, pp213-223. Authors are Glick and
Pon.

Mike Forte

-- 
Mike Forte
forte at ohsu.edu or
forte at mtwo.com



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