In article <32D514EE.7B7B at ryburn.swmed.edu>, Bev Rothermel
<rothermel at ryburn.swmed.edu> wrote:
> I'm looking for information on flow cytometry cell sorting of live
> unfixed yeast cells. Will it work, and does anyone have a good method?
> I want to use GFP as the cell marker and be able to grow up the cells
> after sorting for multiple rounds. The only methods I've seen call for
> fixing the cells prior to FACS.
>> Bev Rothermel
>rothermel at ryburn.swmed.edu
Try Niedenthal et al., Yeast (1996) 12, 773-786.