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cell sorting live yeast

Bev Rothermel rothermel at ryburn.swmed.edu
Thu Jan 9 10:55:26 EST 1997


I'm looking for information on flow cytometry cell sorting of live 
unfixed yeast cells.  Will it work, and does anyone have a good method?  
I want to use GFP as the cell marker and be able to grow up the cells 
after sorting for multiple rounds.  The only methods I've seen call for 
fixing the cells prior to FACS. 

Thanks,

Bev Rothermel
rothermel at ryburn.swmed.edu



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