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fluorogenic/chromogenic invertase substrate??

Sebastian Springer springer at uclink4.berkeley.edu
Wed Jan 8 19:21:04 EST 1997


Invertase, which I want to assay, is a
glucosyl-beta-D-fructofuranosidase. Is anyone aware of a substrate for
this enzyme which would give rise to a fluorescent or coloured product?
There are similar reagents for glucosidases (e.g.
4-nitrophenyl-beta-D-glucoside or fluoresceinyl-beta-D-glucoside) or
mannosidases, but I have found no fructosides in the catalogs. 
Failing that, synthesis advice for a fluoresceinyl fructoside would be
appreciated.

Thanks!,

Sebastian Springer
UC Berkeley
springer at uclink4.berkeley.edu



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