Invertase, which I want to assay, is a
glucosyl-beta-D-fructofuranosidase. Is anyone aware of a substrate for
this enzyme which would give rise to a fluorescent or coloured product?
There are similar reagents for glucosidases (e.g.
4-nitrophenyl-beta-D-glucoside or fluoresceinyl-beta-D-glucoside) or
mannosidases, but I have found no fructosides in the catalogs.
Failing that, synthesis advice for a fluoresceinyl fructoside would be
springer at uclink4.berkeley.edu