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Sequential gene disruption

David Stillman stillman at bioscience.utah.edu
Mon Jan 6 10:41:31 EST 1997

In article <32CBF46E.741B at pop.uky.edu> Chuck Staben, staben at pop.uky.edu
>Other than the hisG-URA3 cassette of Fonzi, are there other sequential
>disruption vectors available?  Such as a URA3/lox cassette?

There is a URA3 disruptor cassette that uses, instead of lox, the
Zygosaccharomyces rouxii site-specific recombinase. See Roca et al "A
hit-and-run system for targeted genetic manipulations in yeast," Nucl
Acids Research 20:4671 (1992). 

David Stillman
stillman at genetics.utah.edu

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