In article <32CBF46E.741B at pop.uky.edu> Chuck Staben, staben at pop.uky.edu
>Other than the hisG-URA3 cassette of Fonzi, are there other sequential
>disruption vectors available? Such as a URA3/lox cassette?
There is a URA3 disruptor cassette that uses, instead of lox, the
Zygosaccharomyces rouxii site-specific recombinase. See Roca et al "A
hit-and-run system for targeted genetic manipulations in yeast," Nucl
Acids Research 20:4671 (1992).
stillman at genetics.utah.edu