I know the subject has been discussed before on this list, but I would
like to know who I should ask for strains and/or constructs allowing the
expression of T7 RNA pol in S. cerevisiae.
I have to transfect transcripts of several constructs in yeas.
Transfecting RNA transcripts in yeast is not much fun, from what I have
heard. My RNA constructs being cloned under the T7 RNA polymerase
promoter, I'd rather transform them with the plasmid than transfect them
with the corresponding transcript.
Thank you very much for your help.
Leiden Institute of Chemistry, Gorlaeus Laboratories.
Leiden University;Einsteinweg 55 / P.O. Box 9502 2300RA, Leiden The
Phone: +31 71 5274400 TeleFax: +31 71 5274537
Department: Phone: +31 71 527