I have been trying, unsuccessfully, to express two human proteins in
yeast. A third human protein expresses very well. I am using a GAL
1-10 promoter for all three, which should be giving huge overexpression.
I have previously considered that the lack of an effective RBS could be
the culprit. This inquiry resulted in the tip that A/T rich sequences
just upstream of the start site help in expression.
Now, I am wondering how much codon bias can affect protein expression.
If there are a number of ill-favored codons in a row (say ones used less
than 10% in normal yeast proteins), is that enough to halt or severely
restrict the amount of protein translated?
I've come across a couple of articles on the effect in E. coli, but are
there any references on the effect of codon bias in yeast?
Department of Genetics