Dennis Templeton wrote:
>>What is the size limitation of cen-ars and 2-micron plasmids in yeast?
>>I need to make a 40 kb episome, high copy number if possible containing a
>viral genome. It needn't shuttle back into e. coli. I've seen such things
>done with YAC's but is there a size limitation on yeast episomes as there
>is in coli episomes?
There is one good reference for your purpose:
Hieter, P. et al. (1985) Cell 40, 381-392.
They studied the chromosome stability regarding circular vs linear, size,
and ARS & CEN, and they found that the more stable a chromosome, the
bigger in size. According to them, you should not have any problem with
CEN plasmids bigger than 100kb.
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