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Size limitation for episomes? 40 kb?

Namjin Chung nc1 at ACPUB.DUKE.EDU
Sat Apr 26 19:30:21 EST 1997

Dennis Templeton wrote:
>What is the size limitation of cen-ars and 2-micron plasmids in yeast? 
>I need to make a 40 kb episome, high copy number if possible containing a
>viral genome. It needn't shuttle back into e. coli. I've seen such things
>done with YAC's but is there a size limitation on yeast episomes as there
>is in coli episomes?
>Thanks, Dennis

There is one good reference for your purpose:

Hieter, P. et al. (1985) Cell 40, 381-392.

They studied the chromosome stability regarding circular vs linear, size, 
and ARS & CEN, and they found that the more stable a chromosome, the 
bigger in size.  According to them, you should not have any problem with 
CEN plasmids bigger than 100kb.  

Good luck,

  Namjin Chung
  Rm. 213 MSRB, Box 3345
  Program in Molecular Cancer Biology
  Duke University Medical Center
  Durham, NC 27710

  nc1 at acpub.duke.edu

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