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Size limitation for episomes? 40 kb?

Dennis Templeton djt2 at po.cwru.edu
Fri Apr 25 15:24:28 EST 1997


What is the size limitation of cen-ars and 2-micron plasmids in yeast? 

I need to make a 40 kb episome, high copy number if possible containing a
viral genome. It needn't shuttle back into e. coli. I've seen such things
done with YAC's but is there a size limitation on yeast episomes as there
is in coli episomes?

Thanks, Dennis

(p.s. if you reply, please copy me by email)

Dennis Templeton
Institute of Pathology
CWRU School of Medicine
djt2 at po.cwru.edu
http://129.22.87.13



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