What is the size limitation of cen-ars and 2-micron plasmids in yeast?
I need to make a 40 kb episome, high copy number if possible containing a
viral genome. It needn't shuttle back into e. coli. I've seen such things
done with YAC's but is there a size limitation on yeast episomes as there
is in coli episomes?
(p.s. if you reply, please copy me by email)
Institute of Pathology
CWRU School of Medicine
djt2 at po.cwru.eduhttp://18.104.22.168