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YPD medium lacking uracil

John McCusker mccusker at ABACUS.MC.DUKE.EDU
Tue Apr 22 08:55:01 EST 1997


Allow me to re-phrase.  A strain that cannot salvage pyrimidines
(5-fluorouracil resistant, e.g. uracil phosphoribosyl transferase deficient
[fur1]) cannot use exogenous pyrimidines regardless of the type of medium
(YEPD or synthetic medium).  It follows that a fur1 strain is completely
dependent for growth upon de novo pyrimidine synthesis.  A fur1 strain that
contains a chromosomal ura3 mutation *and* URA3 on a plasmid is absolutely
dependent for growth on the URA3 plasmid -- it dies if it loses the
plasmid.  This has nothing to do with 5FOA.

*****************************************************************************
John H. McCusker, Asst. Prof.
Dept. of Microbiology, 3020
Duke University Medical Center
Durham, NC 27710

phone:  (919) 681-6744
fax:    (919) 684-8735
e-mail: mccusker at abacus.mc.duke.edu





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