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YPD medium lacking uracil

Ricardo Azpiroz azpiroz at U.ARIZONA.EDU
Mon Apr 21 11:56:46 EST 1997


Add 1% casamino acids to .67% YNB plus glucose. This medium has everything
yeast might need except for adenine, tryptophan and uracil.

On Mon, 21 Apr 1997 *@sbsu1.auckland.ac.nz wrote:

Dear newsgroup readers,
I remember sometime ago hearing about a commercial source of YP(D) medium
lacking uracil (removed somehow).  I have a strain which requires
selection for maintenance of a URA3 plasmid (2 micron), without the growth
limitation imposed by synthetic medium.  If a YPD-uracil product does not
exist, is there another supplement I could add to SC to help the growth of
a slow-growing strain?  Any suggestions would be much appreciated: if you
could please post to my email address as well as the newsgroup, as
messages tend to scroll off our server.
Thanks v. much,
Colin MacDiarmid (macdiarm at sbsu1.auckland.ac.nz)
SBS, Auckland University.



----------------------
Ricardo Azpiroz
Department of Biochemistry
Life Sciences South
University of Arizona
Tucson AZ 85721-0106

azpiroz at U.Arizona.EDU




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