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YPD medium lacking uracil

Yang Doo Suck microbe at DONALD.HANHYO.CO.KR
Mon Apr 21 18:32:20 EST 1997

Check CSM-Ura from BIO101, co. ltd.
That company sells many kind of auxotroph selection media, like CSM-Ura
(complete synthetic medium lacking Ura), CSM-Ade, an so on.

Bytheway I have a question. Does casamino acid have no uracil? If so, 
I hope somebody could inform me a reference or the reason.

Thanks in advance.

Yang, Doo Suck
Hanhyo Institutes of Technology

On Mon, 21 Apr 1997 *@sbsu1.auckland.ac.nz wrote:

> Dear newsgroup readers,
> I remember sometime ago hearing about a commercial source of YP(D) medium
> lacking uracil (removed somehow).  I have a strain which requires
> selection for maintenance of a URA3 plasmid (2 micron), without the growth
> limitation imposed by synthetic medium.  If a YPD-uracil product does not
> exist, is there another supplement I could add to SC to help the growth of
> a slow-growing strain?  Any suggestions would be much appreciated: if you
> could please post to my email address as well as the newsgroup, as
> messages tend to scroll off our server.
> Thanks v. much,
> Colin MacDiarmid (macdiarm at sbsu1.auckland.ac.nz)
> SBS, Auckland University.

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