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YPD medium lacking uracil

Guillaume Stahl stahl at infobiogen.fr
Mon Apr 21 14:38:50 EST 1997

> is there another supplement I could add to SC to help the growth of
> a slow-growing strain?  Any suggestions would be much appreciated:

Try YNB + sugar + 0.5 % casa-amino acids (hydrolysed milk casein, I 
think). This media is lacking ASN, GLN, TRP (all three hydrolysed, 
perhaps other too) and of course ADE and URA.
Most strains should grow as efficiently on those plastes as on YPD !

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