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TX114 extraction of whole yeast?

Fergus Doherty Fergus.Doherty at nottingham.ac.uk
Thu Apr 10 09:42:50 EST 1997

Is it possible to use Triton X114 on whole intact yeast?  That is to do the
phase trick to separate membrane and soluble proteins.  I would rather do
this on fresh yeast than spheroplasts if it works.  Anyone experienc of

Fergus Doherty,
Dept Biochemistry,
Nottingham University,

Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)

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