In article (Dans l'article) <1997Mar24.111601.5821 at news>, Albert Spielmann
<Albert.spielmann at bota.unine.ch> wrote (écrivait) :
> Hi netters,
> I'm working with the two-hybrid system from Clontech.
> The yeast strains are CG1945 and Y190. Both have a ura3-52 deletion.
> Normally they can't grow without uracil. But as said in the supplier's
> protocol, it must grow!! Why?
> Thank you for your help.
> David Humair
The Ura3 marker was used to introduce the lacZ reporter gene, thus both
strains are able to grow without uracil.
Salut Albert..quoi de neuf?
Email:meyer at versailles.inra.fr