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Ura3-52 deletion in Clontech strains

Christian Meyer meyer at versailles.inra.fr
Fri Apr 4 09:38:16 EST 1997

In article (Dans l'article) <1997Mar24.111601.5821 at news>, Albert Spielmann
<Albert.spielmann at bota.unine.ch> wrote (écrivait) :

> Hi netters,
> I'm working with the two-hybrid system from Clontech.
> The yeast strains are CG1945 and Y190. Both have a ura3-52 deletion.
> Normally they can't grow without uracil. But as said in the supplier's
> protocol, it must grow!! Why?
> Thank you for your help.
> David Humair
The Ura3 marker was used to introduce the lacZ reporter gene, thus both
strains are able to grow without uracil.
Christian Meyer
Salut Albert..quoi de neuf?

Christian MEYER
Email:meyer at versailles.inra.fr

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