1) Fix cells for 30 minutes in 70% ethanol.
2) Resuspend in 1 ml 0.1% Nonidet P-40, 10 mM NaCl, 50 mM sodium citrate
pH 7.0. Add Pi to 100 ug/ml. Incubate on benchtop for 30 min.
3) Pellet and wash cells twice, resuspend in 50 mM citrate buffer .
4) View under microscope, stable for at least a few hours. VERY intense.
Try this. Cells are dead of course, so this only works for terminal
experiments. It's essentially a stripped-down flow cytometry protocol.
Kevin A. Morano
Dept. of Biological Chemistry
University of Michigan Medical School
Ann Arbor, MI 48109-0606
kmorano at umich.edu