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Two-hybrid problems

ZX xuez at esvax.dnet.dupont.com
Thu Apr 3 10:17:06 EST 1997


I would also watch for different preys in a single cell.  I had the 
problem before.  It may be a good idea to isolate some 20 to 30 
independent prey plasmids from your positive colony and make sure that 
they are all the same.
Zhixiong Xue
DuPont Central Research
P.O.Box 80402
Wilmington, DE19880-0402
(302) 695-9465

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