I have been having some strange problems with 5-FOA lately. I have
strains with direct repeats of URA3 and ura3 Delta NcoI which, in the
past, papped like crazy on 5-FOA. In the past few months however,
nothing seems to pap well, nor consistently on 5-FOA. I have checked
everything, including using a different stock of 5-FOA from another lab
to no avail. I am absolutely sure that my strains have one wild type
URA3 and one frameshift allele and that they are in direct repeat
orientation. I am stumped why I cannot get them to pap! Any help or
advice would be appreciated.
Dean H. Saxe "Life gives us hurdles, and we must jump,
dsaxe at bimcore.emory.edu and if you fall, you get your ass back up!"
-Millan & Kenzie "Blow"
Graduate Student, Dept. of Genetics & Molecular Biology, Emory Univ.
My home page --- <URL: http://userwww.service.emory.edu/~dsaxe>