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5-FOA problems?

Dean H. Saxe dsaxe at bimcore.emory.edu
Mon Oct 28 16:22:18 EST 1996

I have been having some strange problems with 5-FOA lately.  I have 
strains with direct repeats of URA3 and ura3 Delta NcoI which, in the 
past, papped like crazy on 5-FOA.  In the past few months however, 
nothing seems to pap well, nor consistently on 5-FOA.  I have checked 
everything, including using a different stock of 5-FOA from another lab 
to no avail.  I am absolutely sure that my strains have one wild type 
URA3 and one frameshift allele and that they are in direct repeat 
orientation.  I am stumped why I cannot get them to pap!  Any help or 
advice would be appreciated.


Dean H. Saxe		"Life gives us hurdles, and we must jump,
dsaxe at bimcore.emory.edu  and if you fall, you get your ass back up!"
				-Millan & Kenzie "Blow"
Graduate Student, Dept. of Genetics & Molecular Biology, Emory Univ.
My home page --- <URL: http://userwww.service.emory.edu/~dsaxe>

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