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tagging mutagenesis in yeast?????

Eckhard Boles boles at uni-duesseldorf.de
Thu Oct 24 11:41:28 EST 1996


Hi,

I would like to perform a random mutagenesis with a specific S.
cerevisiae mutant strain selecting for a positive phenotype which enables
me to identify the corresponding gene. Can anyone provide me with
information about tagging mutagenesis systems in yeast, e.g. inducible Ty
transposable elements on a plasmid, which after creating the
insertional mutation allow to identify the mutated gene?

Thanks a lot for your help.
EBo





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