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c-terminal tag for transmembrane protein

rtemple at NETMAIL.HSCBKLYN.EDU rtemple at NETMAIL.HSCBKLYN.EDU
Thu Oct 24 13:05:13 EST 1996


Hello all -

I've got a 12-pass channel protein that I'd like to tag & visualize. I don't
want to mess w/ any signaling on the amino end, and internal tags could mess up
function. After checking the catalogs, I don't see a vector around that will
carboxy-tag commercially and I don't want to 'hand clone' repetitive HA's on the
end unless I have to do so. I do not want to raise Ab's to an integral protein.
Does anyone know of a source of such a vector? Any help would be greatly
appreciated. 

Thanks in advance -
Robyn Temple
SUNY Health Science Center at Brooklyn




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