Eckhard Boles wrote:
>> I would like to perform a random mutagenesis with a specific S.
> cerevisiae mutant strain selecting for a positive phenotype which enables
> me to identify the corresponding gene. Can anyone provide me with
> information about tagging mutagenesis systems in yeast, e.g. inducible Ty
> transposable elements on a plasmid, which after creating the
> insertional mutation allow to identify the mutated gene?
>> Thanks a lot for your help.
Read the paper from Mike Snyder's lab:
Burns et al. 1994. G&D 8, 1087