IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Galactose induction and repression

Daniel Favre favre at medcor.mcgill.ca
Wed Oct 16 14:48:41 EST 1996

Hello everybody,

I have a GFP-fusion protein that can be induced with galactose. My aim is
to generate a pool of this protein, inhibit it's production by growing the
cells in glucose-containing medium, and then analyze the localization of
the protein by fluorescence.
Questions: is a 30 minutes galactose induction sufficient? How long does
it take to repress the expression after the cells have been shifted to
glucose-containing medium? In this respect, what are indeed the optimal

Thank you very much for your attention and comments

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net