Thanks for the responses concerning the gapped-plasmid
PCR co-transformation experiments. I have a new question.
Does anyone know where the sequence of the pRS317 Hieter
plasmid can be downloaded from? It does not seem to be in
NCBI that I can find. If not, does anyone know if it is the
RI to HIII fragment of LYS2 that is blunted into the same
site as the URA3 etc. genes in other Hieter vectors? Thanks
again for your help.