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TRP1 gene counter selection.

Matthew Sachs msachs at ADMIN.OGI.EDU
Tue Oct 15 17:16:29 EST 1996


Trp mutants that accumulate anthranilate will fluoresce blue when
irradiated with long-wave ultraviolet light.  This is the basis for
screening (not selection) methods that are used to find Trp mutants that
accumulate anthranilate.

>There is something that does this in Arabidopsis, and maybe yeast too. It
>was used in Fink's lab to find trp mutants in Arabi. Check out Robert Last
>and G. Fink papers in Plant Cell, late 80's.
>
>
>On Fri, 11 Oct 1996, P.Bundock wrote:
>
>Hi, I urgently need information on any compound that acts as a negative
>selection against the S.cerevisiae TRP1 gene. What I am thinking of is
>something similar to the URA3/5-FOA combination.Does anybody know of such
>a compound and where I would be able to get it? Thnaks a lot.
>
>
>
>
>----------------------
>Ricardo Azpiroz
>Department of Biochemistry
>Life Sciences South
>University of Arizona
>Tucson AZ 85721-0106
>
>azpiroz at U.Arizona.EDU

-----------------------------------------------------------
Matthew Sachs
Department of Chemistry, Biochemistry and Molecular Biology
Oregon Graduate Institute of Science and Technology
20000 NW Walker Road
P.O. Box 91000
Portland, OR  97291-1000
503 690-1487 Phone
503 690-1464 Fax
msachs at admin.ogi.edu





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