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yeast parasite?

Dianne Emslie s9033036 at COUGAR.VUT.EDU.AU
Tue Oct 15 17:22:12 EST 1996

I have a problem which looks like a contaminating organism growing on 
top of my yeast colonies.  These are white, hard "spots" which only 
appear after plates have been at 4deg for a while and on yeast growing on 
ethanol or glycerol/ethanol plates. These "spots" have to be crushed 
to view under the microscope but look like crushed glass in what could be 
a lipid matrix.  Gram stains do not show anything which looks like 
another organism apart from yeast.  The general opinion in my lab is that 
the yeast is producing a lipid or polysaccharide but I would like to be 
sure that there is no contaminant present before I start molecular work.  

Has anyone experienced this kind of thing?  Do yeasts have parasites such 
as viruses? I would be grateful for advice and ideas on what is going on.

Cheers Dianne

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