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Francisco G da Nobrega ib - bio 7588 fgdnobre at usp.br
Thu Oct 10 15:17:59 EST 1996

Dear Brian:

That was nice! What about telling us your conditions that would not only 
increase the yield and save the expensive stuff. Why don't you send this 
to TIGS as a tip to the yeast people?

F.G. Nobrega
Universidade de Sao Paulo
Departamento de Microbiologia - ICB II / USP
Av. Prof. Lineu Prestes, 1374
05508-900   Sao Paulo  -  SP  - Brasil
Voice: (011) 818-7588    Fax: 55-11-818-7354    E-mail:  fgdnobre at usp.br

On 10 Oct 1996, Brian Wickes wrote:

> How's this for an answer;  The reason that YNB and not YPD is preferred 
> is that you want the cells to quickly suck up the FOA.  Although YNB 
> does have plenty of nitrogen, there is no question that cells grow 
> better on YPD and therefore may not take up the FOA as rapidly.  
> Therefore you need more FOA which in turn requires you to ultimately 
> spend more money!  There was a paper put out a few years ago showing 
> that, in fact, if you reduce the amount of added uracil (which allows 
> mutants which will be resistant to FOA to survive) you can use less FOA.  
> I have found that if you do that as well as reduce the level of dextrose 
> you get even more mutants--most likely due to starvation which causes 
> the cells to really crank up the permease activity.
> Brian Wickes

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