That was nice! What about telling us your conditions that would not only
increase the yield and save the expensive stuff. Why don't you send this
to TIGS as a tip to the yeast people?
Universidade de Sao Paulo
Departamento de Microbiologia - ICB II / USP
Av. Prof. Lineu Prestes, 1374
05508-900 Sao Paulo - SP - Brasil
Voice: (011) 818-7588 Fax: 55-11-818-7354 E-mail: fgdnobre at usp.br
On 10 Oct 1996, Brian Wickes wrote:
> How's this for an answer; The reason that YNB and not YPD is preferred
> is that you want the cells to quickly suck up the FOA. Although YNB
> does have plenty of nitrogen, there is no question that cells grow
> better on YPD and therefore may not take up the FOA as rapidly.
> Therefore you need more FOA which in turn requires you to ultimately
> spend more money! There was a paper put out a few years ago showing
> that, in fact, if you reduce the amount of added uracil (which allows
> mutants which will be resistant to FOA to survive) you can use less FOA.
> I have found that if you do that as well as reduce the level of dextrose
> you get even more mutants--most likely due to starvation which causes
> the cells to really crank up the permease activity.
>> Brian Wickes